S. Neifar, F.V. Cervantes, H. BenHlima, A. Bouanane-Darenfed, A.O. Ballesteros, F.J. Plou, S. Bejar. “Immobilization of the glucose isomerase from Caldicoprobacter algeriensis on Sepabeads EC-HA and its efficient application in continuous High Fructose Syrup production using packed bed reactor”Food Chemistry  309,  125710 (2019)doi: 10.1016/j.foodchem.2019.125710


              S. Alonso, G. Santiago, I. Cea-Rama, L. Fernandez-Lopez, C. Coscolin, J. Modregger, A.K. Ressmann, M. Martinez-Martinez, H. Marrero, R. Bargiela, M. Pita, J.L. Gonzalez-Alfonso, M. Briand, D. Rojo, C. Barbas, F.J. Plou, P.N. Golyshin, P. Shahgaldian, J. Sanz-Aparicio, V. Guallar and M. Ferrer. “Genetically engineered proteins with two active sites for enhanced biocatalysis and synergistic chemo- and biocatalysis". Nature Catalysis (2019), doi:10.1038/s41929-019-0394-4

              S. Míguez-Amil, E. Jiménez-Ortega, M. Ramírez-Escudero, D. Talens-Perales, J. Marín-Navarro, J. Polaina, J. Sanz-Aparicio, R. Fernández-Leiro. "The cryo-EM structure of Thermotoga maritima β-galactosidase: quaternary structure guides protein engineering"ACS Chem. Biol. (2019), doi:10.1021/acschembio.9b00752

              M. Ramirez-Escudero, N. Miguez, M. Gimeno-Perez, A.O. Ballesteros, M. Fernandez-Lobato, F.J. Plou and J. Sanz-Aparicio. “Deciphering the molecular specificity of phenolic compounds as inhibitors or glycosyl acceptors of β fructofuranosidase from Xanthophyllomyces dendrorhous. Scientific Reports 9, 17441 (2019), doi:10.1038/s41598-019-53948-y.

              M. Garcia-Gonzalez, F.V. Cervantes, F.J. Plou, M. Remacha, A. Poveda, J. Jiménez-Barbero and M. Fernandez-Lobato. “Efficient production of isomelezitose by a novel glucosyltransferase activity in Metschnikowia reukaufii cell extracts”. Microbial Biotechnology (2019), doi:10.1111/1751-7915.13490

              H. Garcia-Arellano, J. L. Gonzalez-Alfonso, C. Ubilla, F. Comelles, M. Alcalde, M. Bernabé, J-L Parra, A. O. Ballesteros and F. J. Plou. “Production and surfactant properties of tert-butyl α-D-glucopyranosides catalyzed by cyclodextrin glucanotransferase”. Catalysts (2019), .

              M Martínez-Bailén, E Jiménez-Ortega, AT Carmona, I Robina, J Sanz-Aparicio, D Talens-Perales, J Polaina, C Matassini, F Cardonad and AJ Moreno-Vargas. "Structural basis of the inhibition of GH1 β-glucosidases by multivalent pyrrolidine iminosugars"Bioorganic Chemistry 89, 103026-103036 (2019)doi:10.1016/j.bioorg.2019.103026

              P. Santos-Moriano, P. Kidibule, N. Miguez, L. Fernández-Arrojo, A.O. Ballesteros, M. Fernández-Lobato, F.J. Plou. “Tailored enzymatic synthesis of chitooligosaccharides with different deacetylation degrees and their anti-inflammatory activity”. Catalysts (2019), .

              J.L. Gonzalez-Alfonso, P. Peñalver, A.O. Ballesteros, J.C. Morales and F.J. Plou. “Effect of α-glucosylation on the stability, antioxidant properties, toxicity and neuroprotective activity  of (‒)-epigallocatechin gallate”. Frontiers in Nutrition (2019), doi: 10.3389/fnut.2019.00030

              P. Molina-Espeja, P. Santos-Moriano, E. García-Ruiz, A. Ballesteros, FJ. Plou and M. Alcalde. “One-point oriented immobilization of an evolved unspecific peroxygenase”. International Journal of Molecular Sciences (2019), doi:10.3390/ijms20071627 .

              M.S. Gutiérrez, S. Campusano, A.M. Gonzalez, M. Gómez, S. Barahona, D. Sepúlveda, P.J. Espenshade, M. Fernández-Lobato, M. Baeza, V. Cifuentes, J. Alcaíno, J. “Sterol Regulatory Element-Binding Protein (Sre1) promotes the synthesis of carotenoids and sterols in Xanthophyllomyces dendrorhous. Frontiers in Microbiology (2019) doi: 10.3389/fmicb.2019.00586 


              J.L. González-Alfonso, N. Míguez, J. D. Padilla, L. Leemans, A. Poveda, J. Jiménez-Barbero, A. O. Ballesteros, G. Sandoval and F. J. Plou.''Optimization of Regioselective Glucosylation of Hesperetin Catalyzed by Cyclodextrin Glucanotransferase'' . Molecules, 23, 2285, (2018) doi: 10.3390/molecules23112885 

              Baeza M, Fernández-Lobato M, Alcaíno J, Cifuentes V. "Isolation and Characterization of Extrachromosomal Double-Stranded RNA Elements from Carotenogenic Yeasts" . Methods Molecular Biology 1852:327-339 (2018)

              J.L. Gonzalez-Alfonso, L. Casas-Godoy, J. Arrizon, D. Arrieta-Baez, A.O. Ballesteros, G. Sandoval and F.J. Plou. ''Lipase-catalyzed synthesis of fatty acid esters of trisaccharides'' . Methods Molecular Biology, 1835:287-296. (2018), doi:10.1007/978-1-4939-8672-9_15

              D. Rodrigo-Frutos, D. Piedrabuena, J. Sanz-Aparicio, M. Fernández-Lobato. "Yeast cultures expressing the Ffase from Schwanniomyces occidentalis, a simple system to produce the potential prebiotic sugar 6-kestose" Applied Microbiology and Biotechnology (2018), doi:10.1007/s00253-018-9446-y

              P. Santos-Moriano, P.E. Kidibule, E. Alleyne, A.O. Ballesteros, A. Heras, M. Fernandez-Lobato, F.J. Plou. "Efficient conversion of chitosan into chitooligosaccharides by a chitosanolytic activity from Bacillus thuringiensis”.  Process Biochemistry 73, 102-108 (2018). doi:10.1016/j.procbio.2018.07.017

              N. Míguez, M. Ramírez-Escudero, M. Gimeno-Pérez, A. Poveda, J. Jiménez-Barbero, A. O. Ballesteros, M. Fernández-Lobato, J. Sanz-Aparicio and F. J. Plou. “Fructosylation of hydroxytyrosol by the β-fructofuranosidase from Xanthophyllomyces dendrorhous: Insights into the molecular basis of the enzyme specificity. ChemCatChem (2018). doi:10.1002/cctc.201801171

               J.L. Gonzalez-Alfonso, L. Leemans, A. Poveda, J. Jimenez-Barbero, A.O. Ballesteros and F.J. Plou. "Efficient α-glucosylation of epigallocatechin gallate catalyzed by cyclodextrin glucanotransferase from Thermoanaerobacter sp." Journal of Agricultural and Food Chemistry 66, 7402–7408 (2018). doi:10.1021/acs.jafc.8b02143

              J.L. González-Alfonso, D. Rodrigo-Frutos, E. Belmonte-Reche, P. Peñalver, A. Poveda, J. Jimenez-Barbero, A.O. Ballesteros, Y. Hirose, J. Polaina, J.C. Morales, M. Fernández-Lobato and F. J. Plou. "Enzymatic synthesis of a novel pterostilbene α-glucoside by the combination of cyclodextrin glucanotransferase and amyloglucosidase". Molecules 23(6): 1271 (2018). doi:10.3390/molecules23061271

              B. Gomez-Fernandez, E. Garcia-Ruiz, J. Martin, P. Gomez de Santos, P. Santos-Moriano, F.J. Plou, A. Ballesteros, M. Garcia, M. Rodriguez, V.A. Risso, J.M. Sanchez-Ruiz, S.M. Whitney, M. Alcalde. "Directed -in vitro- evolution of Precambrian and extant Rubiscos". Scientific Reports 8, 532 (2018). doi:10.1038/s41598-018-23869-3

              N. Miguez, M. Gimeno-Perez, D. Fernandez-Polo, F.V. Cervantes, A.O. Ballesteros, M. Fernandez-Lobato, M.H. Ribeiro, F.J Plou. "Immobilization of the β-fructofuranosidase from Xanthophyllomyces dendrorhous by entrapment in polyvinyl alcohol and its application to neo-fructooligosaccharides production". Catalysts 8(5), 201 (2018). doi:10.3390/catal8050201

              A.R. Alcántara, E. García-Junceda, V. Gotor-Santamaría, F.J. Plou. "Biocatalysis in Spain: a field of success and innovation". Biocatalysis and Biotransformation (2018).  Special Issue "Biocatalysis in Spain", doi:10.1080/ 10242422.2017.1420064

              P.E. Kidibule,  P. Santos-Moriano, E. Jiménez-Ortega, M. Ramírez-Escudero, M.C. Limón, M. Remacha, F.J. Plou, J. Sanz-Aparicio,  M. Fernández-Lobato. "Use of chitin and chitosan to produce new chitooligosaccharides by chitinase Chit42: enzymatic activity and structural basis of protein specificity". Microbial Cell Factories 17:47 (2018) doi: 10.1186/s12934-018-0895-x

              P. Santos-Moriano, L. Fernandez-Arrojo, M. Mengibar, E. Belmonte-Reche, P. Peñalver, F.N. Acosta, P. Kidibule, A.O. Ballesteros, J.C. Morales, M. Fernandez-Lobato and F.J. Plou. "Enzymatic production of fully deacetylated chitooligosaccharides and their neuroprotective and anti-inflammatory properties". Biocatalysis and Biotransformation 36, 57-67 (2018). Special Issue "Biocatalysis in Spain". doi: 10.1080/10242422.2017.1295231

              M. Ramírez-Escudero, P. Molina-Espeja, P. Gómez de Santos, M. Hofritcher, J. Sanz-Aparicio, M. Alcalde. "Structural insights in the substrate promiscuity of a laboratory-evolved peroxygenase"ACS Chem. Biol. (2018), 13, 3259–3268.  doi:10.1021/acschembio.8b00500


      M. Casa-Villegas, J. Marín-Navarro, J. Polaina. "Synergies in coupled hydrolysis and fermentation of cellulose using a Trichoderma reesei enzyme preparation and a recombinant Saccharomyces cerevisiae strain". World Journal of Microbiology and Biotechnology 33:140 (2017).  doi: 10.1007/s11274-017-2308-4

      A. Rico-Diaz, M. Ramirez-Escudero, A. Vizoso-Vazquez, M.E. Cerdan, M. Becerra, J. Sanz-Aparicio. "Structural features of Aspergillus niger beta-galactosidase define its activity against glycoside linkages"FEBS Journal 284,1815-1829 (2017). doi:10.1111/febs.14083

      L. Deladino, A. Schneider-Teixeira, F.J. Plou, A.S. Navarro, A.D. Molina-García. "Effect of high hydrostatic pressure, alkaline and combined treatments on corn starch granules metal binding: structure, swelling behavior and thermal properties assessment". Food and Bioproducts Processing 102, 241-249 (2017).  doi:10.1016/j.fbp.2017.01.003 

      M. Nieto-Dominguez, L.I. de Eugenio, M.J. York-Durán, B. Rodríguez-Colinas, F.J Plou, E. Chenoll, E. Pardo, F. Codoñer, M.J. Martínez. “Prebiotic effect of xylooligosaccharides produced from birchwood xylan by a novel fungal GH11 xylanase”. Food Chemistry 232, 105-113 (2017), doi:10.1016/j.foodchem.2017.03.149

      M. Casa-Villegas, J. Marín-Navarro, J. Polaina. "Synthesis of isomaltooligosaccharides by Saccharomyces cerevisiae cells expressing Aspergillus niger alpha-glucosidases". ACS Omega 2, 11, 8062-8068 (2017)doi:10.1021/acsomega.7b01189 

    C.V. Yañez-Ñeco, B. Rodriguez-Colinas, L. Amaya-Delgado, A.O. Ballesteros, A.Gschaedler, F.J. Plou, J. Arrizon. "Galactooligosaccharide production from Pantoea anthophila strains isolated from “Tejuino”, a Mexican traditional fermented beverage". Catalysts 7(8), 242 (2017). doi:10.3390/catal7080242

    D. Talens-Perales, J. Marín-Navarro, D. Garrido, E. Almansa, J. Polaina. "Fixation of bioactive compounds to the cuticle of Artemia". Aquaculture 474,  95–100 (2017). doi:10.1016/j.aquaculture.2017.03.044

    M. Nieto-Domínguez, L.I. de Eugenio, P. Peñalver, E. Belmonte-Reche, J.C. Morales, A. Poveda, J. Jimenez-Barbero, A. Prieto, F.J. Plou, M.J. Martínez. "Enzymatic synthesis of a novel neuroprotective hydroxytyrosol glycoside". Journal of Agricultural and Food Chemistry 65, 10526-10533 (2017), doi:10.1021/acs.jafc.7b04176


    S.V. Valenzuela, S. López, P. Biely, J. Sanz-Aparicio, J. Pastor. "The glycoside hydrolase family 8 reducing-end xylose-releasing exo-oligoxylanase Rex8a from Paenibacillus barcinonensis BP-23 is active on branched xylooligosaccharides"Appl. Env. Microbiol. 82, 5116-5124 (2016). doi:10.1128/AEM.01329-16.

    B. Rodriguez-Colinas, L. Fernandez-Arrojo, P. Santos-Moriano, A.O. Ballesteros and F.J. Plou. “Continuous packed bed reactor with immobilized β-galactosidase for production of galactooligosaccharides (GOS)”. Catalysts 6(12), 189 (2016). Special Issue "Immobilized Enzymes: Strategies for Enzyme Stabilization". doi:10.3390/catal6120189

    D. Talens-Perales, A. Górska, D.H. Huson, J. Polaina, J. Marin-
    Navarro. "Analysis of Domain Architecture and Phylogenetics of Family 2 Glycoside Hydrolases (GH2)". PLOS ONE, December, 1-17 (2016)

    Córdova, P., J. Alcaíno, N. Bravo, S. Barahona, D. Sepúlveda, M. Fernández-Lobato, M.Baeza V. Cifuentes. “Regulation of carotenogenesis in the red yeast Xanthophyllomyces dendrorhous: the role of the transcriptional co-repressor complex Cyc8-Tup1 involved in catabolic repression”. Microbial Cell Factories  15(1):193 (2016) doi:10.1186/s12934-016-0597-1Open access

    M. Ramírez-Escudero, M.V. del Pozo, J. Marín-Navarro, B. González, P.N. Golyshin, J. Polaina, M. Ferrer and J. Sanz-Aparicio. "Structural and functional characterization of a ruminal β-glycosidase defines a novel subfamily of glycosyl hydrolase family 3 with permuted domain topology"Journal of Biological Chemistry 291(46):24200-24214 (2016) doi:10.1074/jbc.M116.747527

    I. Saugar, B. Molloy, E. Sanz, M.B. Sánchez, M. Fernández-Lobato, A. Jiménez. "Characterization of the biosynthetic gene cluster (ata) for the A201A aminonucleoside antibiotic from Saccharothrix mutabilis subsp. capreolus". J Antibiot (Tokyo) 70(4):404-413 (2016doi: 10.1038/ja.2016.123

    P. Santos-Moriano, J.M. Woodley, F.J. Plou. "Continuous production of chito-oligosaccharides by an immobilized enzyme in a dual-reactor system"Journal of Molecular Catalysis B: Enzymatic 133, 211-217 (2016)doi:10.1016/j.molcatb.2016.09.001

    P. Santos-Moriano, L. Monsalve-Ledesma, M. Ortega-Muñoz, L. Fernandez-Arrojo, A.O. Ballesteros, F. Santoyo-González, F.J. Plou. "Vinyl sulfone-activated silica for efficient covalent immobilization of alkaline unstable enzymes: application to levansucrase for fructooligosaccharides synthesis". RSC Advances 6, 64175-64181 (2016). doi: 10.1039/C6RA14046G 

    J. Alcaíno , N. Bravo , P. Córdova, A.E. Marcoleta, G. Contreras, S. Barahona, D. Sepúlveda, M. Fernández-Lobato, M. Baeza, V. Cifuentes. "The involvement of Mig1 from Xanthophyllomyces dendrorhous in catabolic repression: An active mechanism contributing to the regulation of carotenoid production". PLOS ONE 11(9):e0162838 (2016). doi:10.1371/journal.pone.0162838 Open access

    D. Piedrabuena, N. Miguez, A. Poveda, F.J. Plou, M. Fernández-Lobato. “Exploring the transferase activity of Ffase from Schwanniomyces occidentalis, a β-fructofuranosidase showing high fructosyl-acceptor promiscuity”. Applied Microbiology and Biotechnology 100(20), 8769-8778 (2016). doi:10.1007/s00253-016-7628-z  

    M. Ramírez-Escudero, M. Gimeno-Pérez, B. González, D. Linde, Z. Merzdo, M. Fernández-Lobato, J. Sanz-Aparicio. "Structural analysis of β-fructofuranosidase from Xanthophyllomyces dendrorhous reveals unique features and the crucial role of N-glycosylation in oligomerization and activity". Journal of Biological Chemistry 291, 6843-6857 (2016). doi: jbc.M115.708495

    D. Talens-Perales, J. Polaina, J. Marín-Navarro. "Structural dissection of the active site of Thermotoga maritima β‑galactosidase identifies key residues for transglycosylating activity".  Journal of Agriculture and Food Chemistry  64, 2917−2924 (2016). doi: 10.1021/acs.jafc.6b00222

    L. Casas-Godoy, J. Arrizon, D. Arrieta, F.J. Plou and G. Sandoval. “Synthesis and emulsifying properties of carbohydrate fatty acid esters produced from Agave tequilana fructans by enzymatic acylation”. Food Chemistry 204, 437-443 (2016). doi:10.1016/j.foodchem.2016.02.153 

    P. Gutierrez-Alonso, M. Gimeno-Pérez, M. Ramírez-Escudero, F.J. Plou, J. Sanz-Aparicio, M. Fernández-Lobato. “Molecular characterization and heterologous expression of a Xanthophyllomyces dendrorhous α-glucosidase with potential for prebiotics production”Applied Microbiology and Biotechnology 100(7), 3125-3135

    P. Zambelli, L. Tamborini, S. Cazzamalli, A. Pinto, S. Arioli, S. Balzaretti, F.J. Plou, L. Fernandez-Arrojo, F. Molinari, P. Conti, D. Romano. “An efficient continuous flow process for the synthesis of a non-conventional mixture of fructooligosaccharides”Food Chemistry 190, 607-613 (2016)  doi:10.1016/j.foodchem.2015.06.002


    J. Marín-Navarro, N. Roupain, D. Talens-Perales, J. Polaina. "Identification and structural analysis of amino acid substitutions that increase the stability and activity of Aspergillus niger glucose oxidase". PLOS ONE 10(12): e0144289 (2015) doi: 10.1371/journal.pone.0144289
      Open access

    P. Santos-Moriano, L. Fernandez-Arrojo, A. Poveda, J. Jimenez-Barbero, A.O. Ballesteros and F.J. Plou. “Levan versus fructooligosaccharide synthesis using the levansucrase from Zymomonas mobilis: effect of reaction conditions”. Journal of Molecular Catalysis B: Enzymatic, 119, 18–25 (2015), doi:10.1016/j.molcatb.2015.05.011 

    M.A. Sainz-Polo, B. González, M. Menéndez, F.I.J. Pastor and J. Sanz-Aparicio. "Exploring Multimodularity in Plant Cell Wall Deconstruction: Structural and Functional Analysis of Xyn10C Containing the CBM22-1-CBM22-2 Tandem". Journal of Biological Chemistry 290(28):17116-30 (2015), doi:10.1074/jbc.M115.659300

    P. Zambelli, I. Serra, L. Fernandez-Arrojo, F.J. Plou, L. Tamborini, P. Conti, M.I. Contente, F. Molinari, D. Romano. “Sweet-and-salty biocatalysis: fructooligosaccharides production using Cladosporium cladosporioides in seawater”. Process Biochemistry 50, 1086-1090 (2015), doi:10.1016/j.procbio.2015.04.006 

    M.A. Sainz-Polo, B. González-Pérez, F.J. Pastor, J. Sanz-Aparicio."Crystallization and preliminary X-ray diffraction analysis of the N-terminal domain of Paenibacillus barcinonensis xylanase 10C containing the CBM22-1--CBM22-2 tandem". Acta Crystallogr. F71, 136-140 (2015). doi:10.1107/S2053230X14027496 

    J. Marín-Navarro, D. Talens and J. Polaina. "One-pot production of fructooligosaccharides by a Saccharomyces cerevisiae strain expressing an engineered invertase". Applied Microbiology and Biotechnology 99(6):2549-2555

    P. Santos-Moriano, L. Fernández-Arrojo, B. Rodríguez-Colinas, A.O. Ballesteros, F.J. Plou. "Enzymatic synthesis of fructooligosaccharides that stimulate the gut microbiota". Anales de la Real Academia Nacional de Farmacia  81, 48-62 (2015). Download

    J. Sanz-Aparicio. “The legacy of women to crystallography”. Arbor 191 (772): a223 (2015). doi:10.3989/arbor.2015.772n2002. Special Issue "Celebrating 100 years of modern crystallography" (Mancheño, J.M., Ed..)

    L. Fernandez-Arrojo, P. Santos-Moriano, B. Rodriguez-Colinas, A.O. Ballesteros and F.J. Plou. "Micro-scale procedure for enzyme immobilization screening and operational stability assays". Biotechnology Letters 37, 1593-1600 

    N. Corzo, J. L. Alonso, F. Azpiroz, M. A. Calvo, M. Cirici, R. Leis, F. Lombó, I. Mateos-Aparicio, F. J. Plou, P. Ruas-Madiedo, P. Ruperez, A. Redondo-Cuenca, M. L. Sanz and A. Clemente. Prebióticos; concepto, propiedades y efectos beneficiosos. Nutrición Hospitalaria 31 (Supl. 1), 99-118 (2015). Download   

    M. Gimeno, D. Linde, L. Fernández-Arrojo, F.J. Plou, M. Fernández Lobato. Heterologous over-production of β-fructofuranosidase from yeast Xanthophyllomyces dendrorhous, an enzyme producing prebiotic sugars. Applied Microbiology and Biotechnology 99, 3459-3467 (2015),


    P. Zambelli, L. Fernandez-Arrojo, D. Romano, P. Santos-Moriano, M. Gimeno-Perez, A. Poveda, R. Gandolfi, M. Fernández-Lobato, F. Molinari, F.J. Plou. Production of fructooligosaccharides by mycelium-bound transfructosylation activity present in Cladosporium cladosporioides and Penicilium sizovae. Process Biochemistry 49, 2174-2180 (2014).   

    B. Rodriguez-Colinas, L. Fernandez-Arrojo, A.O. Ballesteros, F.J. Plou. Galactooligosaccharides formation during enzymatic hydrolysis of lactose: towards a prebiotic enriched milk.  Food Chemistry, 145, 388-394 (2014)  Postprint Digital CSIC

    Sainz-Polo, M.A.Valenzuela, S., González-Pérez, B., Pastor, F.J., Sanz-Aparicio, J. Structural analysis of glucuronoxylan specific Xyn30D and its attached CBM35 domain give insights into the role of modularity in specificity. Journal of Biological Chemistry 289, 31088-31101 (2014).

    Rico-Díaz, A., Vizoso-Vázquez, A-, Cerdán, M.A., Becerra, M., Sanz-Aparicio, J. Crystallization and preliminary X-ray diffraction data of β-galactosidase from Aspergillus nigerActa Crystallogr F70, 1529-1531 (2014)

    Castruita-Domínguez, J.P., González-Hernández, S.E., Polaina, J., Flores-Villavicencio, L.L., Alvarez-Vargas, A., Flores-Martínez, A., Ponce-Noyola, P., Leal-Morales, C.A.. Analysis of a polygalacturonase gene of Ustilago maydis and characterization of the encoded enzyme. Journal of Basic Microbiology 54, 340-349 (2014).

    Marín-Navarro, J., Talens-Perales, D., Oude-Vrielink, A., Cañada, F.J., Polaina, J. Immobilization of thermostable β-galactosidase on epoxy support and its use for lactose hydrolysis and galactooligosaccharides biosynthesisWorld Journal of Microbiology and Biotechnology 30989-998 (2014).

    Sainz-Polo, M.A., Valenzuela, S.V., Pastor, F.J., Sanz-Aparicio, J. Crystallization and preliminary X-ray diffraction analysis of Xyn30D from Paenibacillus barcinonensis. Acta Crystallographica Section F: Structural Biology Communications 70, 963-966 (2014).
    M. Gimeno-Perez, P. Santos-Moriano, L. Fernández-Arrojo, A. Poveda, J. Jiménez-Barbero, A.O. Ballesteros, M. Fernández-Lobato, F.J. PlouRegioselective synthesis of neo-erlose by the β-fructofuranosidase from Xanthophyllomyces dendrorhousProcess Biochemistry 49, 423-429 (2014). http:/ 


    Castruita-Domínguez JP, González-Hernández SE, Polaina J, Flores-Villavicencio LL, Alvarez-Vargas A, Flores-Martínez A, Ponce-Noyola P, Leal-Morales CA. Analysis of a polygalacturonase gene of Ustilago maydis and characterization of the encoded enzyme. Journal of Basic Microbiology (2013). 

    Álvaro-Benito, M., Fernández-Lobato, M., Baronian, K., Kunze, G. Assessment of Schwanniomyces occidentalis as a host for protein production using the wide-range Xplor expression platform. Applied Microbiology and Biotechnology , 97, 4443 - 4456 (2013)doi: 10.1007/s00253-012-4527-9

    M. de Abreu, M. Alvaro-Benito, J. Sanz-Aparicio, F.J. Plou, M. Fernandez-Lobato, M. Alcalde. Synthesis of 6-kestose improved by a highly efficient β-fructofuranosidase engineered by directed evolution. Advanced Synthesis & Catalysis, 355, 1698-1702 (2013) doi:10.1002/adsc.201200769. 

    L. Fernandez-Arrojo, B. Rodriguez-Colinas, P. Gutierrez-Alonso, M. Fernandez-Lobato, M. Alcalde, A.O. Ballesteros and F.J. Plou. Dried alginate-entrapped enzymes (DALGEEs) and their application to the production of fructooligosaccharides. Process Biochemistry, in press accepted manuscript(2013). doi:10.1016/j.procbio.2013.02.015 

    P. Urrutia, B. Rodriguez-Colinas, L. Fernandez-Arrojo, A.O. Ballesteros, L. Wilson, A. Illanes and F.J. Plou Detailed analysis of galactooligosaccharides synthesis with β-galactosidase from Aspergillus oryzae. Journal of Agricultural and Food Chemistry61, 1081–1087 (2013). doi: 10.1021/jf304354u

    Sainz-Polo, M.A., Ramírez, M., Lafraya, A., González, B., Marín-Navarro, J., Polaina, J., Sanz-Aparicio, J. The three-dimensional structure of Saccharomyces invertase: role of a non-catalytic domain in oligomerization and substrate specificityJournal of Biological Chemistry288, 9755-9766 (2013)  doi:10.1074/jbc.M112.446435. 

    B. Rodriguez-Colinas, S. Kolida, M. Baran, A.O. Ballesteros, R.A. Rastall, F.J. Plou. Analysis of fermentation selectivity of purified galacto-oligosaccharides by in vitro human faecal fermentation. Applied Microbiology and Biotechnology, 97, 5743-5752 (2013)


    M. Alvaro-Benito, M.A. Sainz-Polo, D. Gonzalez-Perez, B. Gonzalez, F.J. Plou, M. Fernandez-Lobato, J. Sanz-AparicioStructural and kinetic insights reveal that the amino acid pair Gln-228/Asn-254 modulates the transfructosylating specificity of Schwanniomyces occidentalis β-fructofuranosidase, an enzyme that produces prebiotics. Journal of Biological Chemistry, 287, 19674-19686 (2012) doi:10.1074/jbc.M112.355503.

    Sainz-Polo, M.A., Lafraya, A., Polo, A., Marín-Navarro, J., Polaina, J., Sanz-Aparicio, J.. Crystallization and preliminary X-ray diffraction analysis of the invertase from Saccharomyces cerevisiaeActa Crystallographica Section F: Structural Biology and Crystallization Communications, Vol. 68, 1538-1541 (2012) doi: 10.1107/S1744309112044417 

    D. Linde, B. Rodriguez-Colinas, M. Estevez, A. Poveda, F.J. Plou and M. Fernandez-Lobato. Analysis of neofructooligosaccharides production mediated by the extracellular beta-fructofuranosidase from Xanthophyllomyces dendrorhous. Bioresource Technology, 23,123-130(2012) doi: 10.1016/j.biortech.2012.01.023 

    Pereira-Rodríguez, A; Fernández-Leiro, R; González-Siso, MI; Esperanza Cerdán, M; Becerra, M; Sanz-Aparicio, J. Structural basis of specificity in tetrameric Kluyveromyces lactis β-galactosidase. Journal of Structural Biology (2012) 177, 392–401.  doi:10.1016/j.jsb.2011.11.031 

    B. Rodriguez-Colinas, A. Poveda, J. Jimenez-Barbero, A.O. Ballesteros, F.J. Plou.Galacto-oligosaccharide synthesis from lactose solution or skim milk using the β-galactosidase from Bacillus circulans. Journal of Agricultural and Food Chemistry60, 6391-6398 (2012)

    Camarillo-Cadena M, Garza-Ramos G, Peimbert M, Polaina J, Pérez-Hernández G, Zubillaga RA. Additive effect of single amino acid replacements on the kinetic stability of ß-glucosidase BThe Protein Journal 31:615-622. (2012) doi: 10.1007/s10930-012-9445-2

    Del Pozo MV, Fernández-Arrojo L, Gil-Martínez J, Montesinos A, Chernikova TN, Nechitaylo TY, Waliszek A, Tortajada M, Rojas A, Huws SA, Golyshina OV, Newbold CJ, Polaina J, Ferrer M, Golyshin PN. Microbial β-glucosidases from cow rumen metagenome enhance the saccharification of lignocellulose in combination with commercial cellulase cocktail.Biotechnol Biofuels. 5(1):73 (2012). doi: 10.1186/1754-6834-5-73. 

    N.R. Aimaretti, C.V. Ybalo, M.L. Rojas, F.J. Plou, J.C. Yori. Production of bioethanol from carrot discards. Bioresource Technology123, 727-732(2012). doi:10.1016/j.biortech.2012.08.035. 

    Ferrer M, Ghazi A, Beloqui A, Vieites JM, López-Cortés N, Marín-Navarro J, Nechitaylo TY, Guazzaroni ME, Polaina J, Waliczek A, Chernikova TN, Reva ON, Golyshina OV, Golyshin PN Functional metagenomics unveils a multifunctional glycosyl hydrolase from the family 43 catalysing the breakdown of plant polymers in the calf rumenPLoS One 7(6):e38134(2012) doi:10.1371/journal.pone.0038134 


    B. Rodriguez-Colinas, M. de Abreu, L. Fernandez-Arrojo, R. de Beer, A. Poveda, J. Jimenez-Barbero, D. Haltrich, A. Ballesteros, M. Fernandez-Lobato and F.J. Plou.Production of galacto-oligosaccharides by the beta-galactosidase fromKluyveromyces lactis: comparative analysis of permeabilized cells versus soluble enzyme. Journal of Agricultural and Food Chemistry, 59, 10477-10484 (2011) doi:10.1021/jf2022012

    Lafraya A, Sanz-Aparicio J, Polaina J, Marin-Navarro J. Fructo-oligosaccharide synthesis by mutant versions of Saccharomyces cerevisiae invertase. Applied and Environmental Microbiology 77 (17) , 6148-6157 (2011)doi: 10.1128/AEM.05032-11

    M.A. de Abreu, M. Álvaro-Benito, F.J. Plou, M. Fernández-Lobato and M. Alcalde.Screening beta-fructofuranosidases mutant libraries to enhance the transglycosylation rates of beta-(2→6) fructooligosaccharides.Combinatorial Chemistry & High-Throughput Screening, 14, 730-738 (2011)doi:10.2174/138620711796504370 

    Marín-Navarro J, Polaina J. Glucoamylases: structural and biotechnological aspectsApplied Microbiology and Biotechnology. 89: 1267-1273 (2011) doi:10.1007/s00253-010-3034-0 

    P. Torres-Salas, A. del Monte-Martinez, B. Cutiño-Avila, B. Rodriguez-Colinas, M. Alcalde, A. O. Ballesteros, F. J. PlouImmobilized biocatalysts: novel approaches and tools for binding enzymes to supports. Advanced Materials, 23, 5275–5282 (2011) doi:10.1002/adma.201101821

    Gurgu, L., Lafraya, A., Polaina, J., Marín-Navarro, J. Fermentation of cellobiose to ethanol by industrial Saccharomyces strains carrying the β-glucosidase gene (BGL1) from Saccharomycopsis fibuligera.Bioresource Technology, 102, 5229-5236 (2011)doi:10.1016/j.biortech.2011.01.062 

    C. Guerrero, C. Vera, F. Plou and A. Illanes. Influence of reaction conditions on the selectivity of the synthesis of lactulose with microbial β-galactosidases. Journal of Molecular Catalysis B: Enzymatic, 72, 206-212 (2011) doi:10.1016/j.molcatb.2011.06.007 

    Marín-Navarro J, L Gurgu, S Alamar and J Polaina. Structural and functional analysis of hybrid enzymes generated by domain shuffling between Saccharomyces cerevisiae (var. diastaticus) Sta1 glucoamylase andSaccharomycopsis fibuligera Bgl1 ß-glucosidase. Applied Microbiology and Biotechnology 89:121-130 (2011) doi:10.1007/s00253-010-2845-3 


    Gallardo O, Pastor FIJ, Polaina J, Díaz P, Isorna P, Gonzalez-Perez B, Sanz-Aparicio J. Structural insights into the specificity of Xyn10B fromPaenibacillus barcinonensis and its improved stability by forced protein evolution. Journal of Biological Chemistry 285, 2721-2733 (2010).doi:10.1074/jbc.M109.064394 

    Alvaro-Benito, M., De Abreu, M., Portillo, F., Sanz-Aparicio, J.,Fernández-Lobato, M. New insights into the fructosyltransferase activity ofSchwanniomyces occidentalis β-fructofuranosidase, emerging from nonconventional codon usage and directed mutation. Applied and Environmental Microbiology 76 (22) , pp. 7491-7499 (2010).doi:10.1128/AEM.01614-10 

    Pereira-Rodríguez, Á., Fernández-Leiro, R., González Siso, M.I., Cerdán, M.E., Becerra, M., Sanz-Aparicio, J. Crystallization and preliminary X-ray crystallographic analysis of beta-galactosidase from Kluyveromyces lactis. Acta Crystallographica Section F: Structural Biology and Crystallization Communications 66 (3) , pp. 297-300 (2010). doi: 10.1107/S1744309109054931

    Polo, A., Linde, D., Estévez, M., Fernández-Lobato, M. And Sanz-Aparicio, J.Crystallization and preliminary X-ray diffraction analysis of the fructofuranosidase from Xanthophyllomyces dendrorhous. Acta Crystallographica F66, 1441-1444 (2010). doi: 10.1107/S1744309110029192

    Fernandez-Leiro, R.,  Pereira-Rodríguez, A., Cerdan, M.E., Becerra, M. and  Sanz-Aparicio, J. Structural analysis of Saccharomyces cerevisiae α-galactosidase and its complexes with natural substrates reveals new insights into substrate specificity of GH27 glycosidasesJournal of Biological Chemistry (2010)doi: 10.1074/jbc.M110.144584