C.M. Curieses-Andrés, J.M. Pérez de la Lastra, C. Andrés-Juan, F.J. Plou, E. Pérez-Lebeña. 
        “Polyphenols as antioxidant/pro-oxidant compounds and donors of reducing species: relationship with human antioxidant metabolism”. Processes 11(9), 2771 (2023)

        C. Figueiredo, A. García-Ortega, T. Mandal, A. Lielpetere, F. Cervantes, D. Demurtas, E. Magner, F.J. Plou, W. Schuhmann, D. Leech, M. Pita, A.L. De Lacey. "An oxygen-insensitive amperometric galactose biosensor based on galactose oxidase co-immobilized with an Os-complex modified redox polymer". Electrochimica Acta 472, 143438 (2023)

        L. Fernandez-Lopez, I. Cea-Rama, J. Alvarez-Malmagro, A.K. Ressmann, J.L. Gonzalez-Alfonso, C. Coscolín, P. Shahgaldian, F.J. Plou, J. Modregger, M. Pita, J. Sanz-Aparicio and M. Ferrer. "Transforming an esterase into an enantioselective chatecholase through bioconjugation of a versatile metal-chelating inhibitor". Chem. Commun. 59(62), 9469-9472 (2023)

        A. Robles-Martín,  R.  Amigot-Sánchez,  L.  Fernandez-Lopez,  J.L.  Gonzalez-Alfonso,  S. Roda,  V.  Alcolea-Rodriguez,  D. Heras-Márquez,  D. Almendral,  C.  Coscolín,  F.J.  Plou,  R. Portela,  M.A.  Bañares,  A. Martínez-del-Pozo,  S. García-Linares,  M.  Ferrer  &  V.  Guallar.  "Sub-micro-  and  nano-sized  polyethylene  terephthalate  deconstruction  with  engineered  protein  nanopores". Nature Catalysis 6, 1174-1185 (2023)

        C.M. Curieses-Andrés, J.M. Perez de Lastra, C. Andres-Juan, F. J. Plou, and E. Pérez-Lebeña. "From reactive species to disease development: Effect of oxidants and antioxidants on the cellular biomarkers". Journal of Biochemical and Molecular Toxicology 37, e23455 (2023)

        M.A. Distaso, T.N. Chernikova, R. Bargiela, C. Coscolín, P. Stogios, J.L. Gonzalez-Alfonso, S.  Lemak, A.N. Khusnutdinova, F.J. Plou, E. Evdokimova, A. Savchenko, E.A. Lunev, M.M. Yakimov, O.V. Golyshina, M. Ferrer, A.F. Yakunin, P.N. Golyshin. "Thermophilic Carboxylesterases from Hydrothermal Vents of the Volcanic Island of Ischia Active on Synthetic and Biobased Polymers and Mycotoxins". Environmental Microbiology, 89, e01704-22 (2023)

        M. Garcia-Gonzalez, E. Narmontaitea, F. V. Cervantes, F. J. Plou, L. Betancor, M. Fernandez-Lobato. "Continuous production of honey oligosaccharides in packed-bed reactors with immobilized α-glucosidase from Metschnikowia reukaufii"Biocatalysis and Agricultural Biotechnology, 47, 102590 (2023)  doi:10.1016/j.bcab.2022.102590

        M. Garcia-Gonzalez, E. Narmontaite, F.V. Cervantes, F.J. Plou, L. Betancor, M. Fernández-Lobato. “Continuous production of honey oligosaccharides in packed-bed reactors with immobilized α-glucosidase from Metschnikowia reukaufiiBiocatalysis and Agricultural Biotechnology 47,  102590 (2023) doi:10.1016/j.bcab.2022.102590


      Capecchi, E., Tomaino, E., Piccinino, D., Kidibule, P.E., Fernandez Lobato, M., Spinelli, D., Pogni, R., Cabado, A., Lago, J., Saladino, R. "Nanoparticles of lignin and saccharides from fishery wastes as sustainable UV-shielding, antioxidant and antimicrobial bio-fillers". Biomacromolecules, 23, 8, 3154–3164 (2022) doi:10.1021/acs.biomac.2c00236

      E. Jiménez-Ortega, E. Narmontaite, B. González-Pérez, F.J. Plou, M. Fernández-Lobato, J. Sanz-Aparicio. “Insights into the structure of the highly-glycosylated Ffase from Rhodotorula dairenensis enhance its biotechnological potential”. International Journal of Molecular Sciences 23(23), 14981 (2022) doi:10.3390/ijms232314981 

      I. Cea-Rama, C. Coscolin, J.L. Gonzalez-Alfonso, J. Raj, M. Vasiljević, F.J. Plou, M. Ferrer, J. Sanz-Aparicio. Crystal structure of a family VIII β-lactamase fold hydrolase reveals the molecular mechanism for its broad substrate scope. FEBS Journal (2022), doi:10.1111/febs.16554

      M. García-González, F. V. Cervantes, R. P. Ipiales, A. de la Rubia, F. J. Plou, M. Fernández-Lobato. "Isomelezitose Overproduction by Alginate-Entrapped Recombinant E. coli Cells and In Vitro Evaluation of Its Potencial Prebiotic Effect"International Journal of Molecular Sciences 23, 12682 (2022) doi:10.3390/ijms232012682 

      N. Miguez, D. Fernández-Polo, P. Santos-Moriano, B. Rodríguez-Colinas, A. Poveda, J. Jiménez-Barbero, A.O. Ballesteros, F.J. Plou. “Enzymatic bioconversion of beechwood xylan into the antioxidant 2’-O-α-(4-O-methyl-D-glucuronosyl)-xylobiose”Biomass Conversion and Biorefinery (2022), doi:10.1007/s13399-022-03240-3

      A. Braga, D. Gomes, J. Rainha, B.B. Cardoso, C. Amorim, S.C. Silvério, M. Fernández-Lobato, J.L. Rodrigues, L.R. Rodrigues. “Tailoring fructooligosaccharides composition with engineered Zymomonas mobilis ZM4”. Applied Microbiology and Biotechnology 106, 4617–4626 (2022). doi:10.1007/s00253-022-12037-3

      J. M. Pérez de la Lastra, C. Andrés Juan, F. J. Plou  and E. Pérez-Lebeña. "Nitration of Flavonoids and Tocopherols as Potential Modulators of Nitrosative Stress—A Study Based on Their Conformational Structures and Energy Content". Stresses 2(2), 213-230 (2022) doi: 10.3390/stresses2020015

      E. Jiménez-Ortega, P.E. Kidibule, M. Fernández-Lobato and J. Sanz-Aparicio. “Structure-function insights into the fungal endo-chitinase Chit33 depict its mechanism on chitinous material”. International Journal of Molecular Sciences 23 (14), 7599 (2022). doi:10.3390/ijms23147599

      S. Roda, L. Fernandez-Lopez, M. Benedens, A. Bollinger, S. Thies, J. Schumacher, C. Coscolín, M. Kazemi, G. Santiago, C.G.W. Gertzen, J.L. Gonzalez-Alfonso, F.J. Plou, K.-E. Jaeger, S.H.J. Smits, M. Ferrer, V. Guallar. “A PluriZyme with transaminase and hydrolase activity catalyzes cascade reactions".  Angewandte Chemie 61(37), e202207344 (2022), doi:10.1002/anie.202207344

      F.V. Cervantes, D. Fernandez-Polo, Z. Merdzo, N. Miguez, M. Garcia-Gonzalez, A.O. Ballesteros, M. Fernandez-Lobato and F-J. Plou. "Reuse of Immobilized Komagataella phaffii Cells for the Elimination of D-Glucose in Syrups of Bioactive Carbohydrates". ACS Food Science Technology 2(4), 682–690 (2022), doi:10.1021/acsfoodscitech.2c00008 


      L. Fernandez-Lopez, S. Sanchez-Carrillo, A. García-Moyano, E. Borchert, D. Almendral, S. Alonso, I. Cea-Rama, N. Miguez, Ø. Larsen, J. Werner, K. S .Makarova, F. J. Plou, T. G. Dahlgren, J. Sanz-Aparicio, U. Hentschelc, G.Elin Kjæreng Bjerg and M. Ferrera. "The bone-degrading enzyme machinery: from multi-component understanding to the treatment of residues from the meat industry". Computational and Structural Biotechnology Journal  19, 6328-6342 (2021), doi:10.1016/j.csbj.2021.11.027

      E  Jiménez-Ortega, PE Kidibule, M Fernández-Lobato, J Sanz-Aparicio.  "Structural inspection and protein motions modelling of a fungal glycoside hydrolase family 18 chitinase by crystallography depicts dynamic enzymatic mechanism". Comput. Struct. Biol. J. (2021). 19, 5466-5478. doi: 10.1016/j.csbj.2021.09.027

      J. A. Méndez-Líter ,I. Ayuso-Fernández ,F. Csarman ,L. Isabel de Eugenio,N. Míguez ,F. J. Plou ,A. Prieto ,R. Ludwig  and M. J. Martínez."Lytic Polysaccharide Monooxygenase from Talaromyces amestolkiae with an Enigmatic Linker-like Region: The Role of This Enzyme on Cellulose Saccharification". International Journal of Molecular Sciences (2021) 22(24), 13611; doi:10.3390/ijms222413611

      J.L. González-Alfonso, A. Poveda, M. Arribas, Y. Hirose, M. Fernández-Lobato, A.O. Ballesteros, J. Jiménez-Barbero, and F.J. Plou. "Polyglucosylation of Rutin Catalyzed by Cyclodextrin Glucanotransferase from Geobacillus sp.: Optimization and Chemical Characterization of Products". Industrial & Engineering Chemistry Research (2021), doi:10.1021/acs.iecr.1c03070

      J.M. Pérez de la Lastra, C. Andrés-Juan, F.J. Plou and E. Pérez-Lebeña. "Theoretical Three-Dimensional Zinc Complexes with Glutathione, Amino Acids and Flavonoids". Stresses (2021),  doi: 10.3390/stresses1030011

      D. Talens-Perales, E. Jiménez-Ortega, P. Sánchez-Torres, J.Sanz-Aparicio, J. Polaina. "Phylogenetic, functional and structural characterization of a GH10 xylanase active at extreme conditions of temperature and alkalinity". Comput. Struct. Biol. J.  19, 2676-2686 (2021) doi: 10.1016/j.csbj.2021.05.004

      D. Piedrabuena, Á. Rumbero, E. Pires, A. Leal-Duaso ,C. Civera, M. Fernández-Lobato, M.J, Hernaiz ."Enzymatic synthesis of novel fructosylated compounds by beta-fructofuranosidase from Schwanniomyces occidentalisin green solvents". RSC Advances, 11, 39, 24312-24319 (2021), doi: 10.1039/d1ra01391b
      W. Snoch, D. Wnuk, T. Witko, J. Staroń, A. J. Bojarski, E. Jarek , F.J. Plou and Maciej Guzik. "In Search of Effective Anticancer Agents—Novel Sugar Esters Based on Polyhydroxyalkanoate Monomers" International Journal of Molecular Sciences (2021), 22(13), 7238,  doi: 10.3390/ijms22137238

      C. Andrés-Juan, J. M. Pérez de la Lastra  , F. J. Plou  and E. Pérez-Lebeña."The Chemistry of Reactive Oxygen Species (ROS) Revisited: Outlining Their Role in Biological Macromolecules (DNA, Lipids and Proteins) and Induced Pathologies". International Journal of Molecular Sciences (2021), 22, 4642,  doi: 10.3390/ijms22094642

      J.L. González-Alfonso, Z. Ubiparip, E. Jiménez-Ortega, A. Poveda, C. Alonso, L. Coderch, J. Jiménez-Barbero, J. Sanz-Aparicio, A. Ballesteros, T. Desmet and F.J. Plou. "Enzymatic Synthesis of Phloretin α‐Glucosides using a Sucrose Phosphorylase Mutant and its Effect on Solubility, Antioxidant Properties and Skin Absorption". Advanced Synthesis & Catalysis 363,  3079-3089 (2021), doi: 10.1002/adsc.202100201

      M. Gimeno-Pérez, Z. Merdzo, E. Castillo-Rosa, C.M. de Hijas and M. Fernández-Lobato. "The β-fructofuranosidase from Rhodotorula dairenensis: Molecular cloning, heterologous expression, and evaluation of its transferase activity". Catalysts Volume 11, Issue 4, (2021), Article number 476, doi: 10.3390/catal11040476

      N. Míguez ,P. Kidibule ,P. Santos-Moriano ,A. O. Ballesteros ,M. Fernández-Lobato and F. J. Plou. "Enzymatic Synthesis and Characterization of Different Families of Chitooligosaccharides and Their Bioactive Properties".  Applied Sciences (2021), 11(7), 3212.  doi: 10.3390/app11073212

      C. Rubio, E. Lizárraga, D. Álvarez-Cilleros, P.  Pérez-Pardo, P. Sanmartin-Salinas, M.V. Toledo-Lobo, C. Álvarez, F. Escrivá, M. Fernández-Lobato, L.G. Guijarro, A.M. Valverde and J.M. Carrascosa. “Aging in male Wistar rats associates with changes in intestinal microbiota, gut structure and cholecystokinin-mediated gut-brain axis function”Journal Gerontology: Serie A, glaa313 (2021)doi: 10.1093/gerona/glaa313

      D. Rodrigo-Frutos, E. Jiménez-Ortega, D. Piedrabuena, M. Ramírez-Escudero, N. Míguez, F. J. Plou, J. Sanz-Aparicio and M. Fernández-Lobato. "New insights into the molecular mechanism behind mannitol and erythritol fructosylation by β-fructofuranosidase from Schwanniomyces occidentalis." Scientific Reports, 11, 7158 (2021), doi: 10.1038/s41598-021-86568-6 

      A. R. Alcántara and F. J. Plou. "Special Issue on “Applied Biocatalysis in Europe: A Sustainable Tool for Improving Life Quality”" Catalysts (2021), 11(3), 339; doi: 10.3390/catal11030339

      P. E. Kidibule, J. Costa, A. Atrei, F. J. Plou, M. Fernández-Lobato and R. Pogni. "Production and characterization of chitooligosaccharides by the fungal chitinase Chit42 immobilized on magnetic nanoparticles and chitosan beads: selectivity, specificity andimproved operational utility". 
      RSC Advances, (2021), 11, 5529, doi: 10.1039/d0ra10409d
        C.V. Yáñez-Ñeco, F.V. Cervantes, L. Amaya-Delgado, A.O. Ballesteros, F.J. Plou nad J. Arrizón. “Synthesis of β(1→3) and β(1→6) galactooligosaccharides from lactose and whey using a recombinant β-galactosidase from Pantoea anthophilaElectronic Journal of Biotechnology 49, 14-21 (2021)doi: 10.1016/j.ejbt.2020.10.004


             M. García-González, M. Minguet-Lobato, F.J. Plou, M. Fernández-Lobato.“Molecular characterization and heterologous expression of two alpha-glucosidases from Metschnikowia spp, both producers of honey sugars”. Microbial Cell Factories 19, 140 (2020) doi: 10.1186/s12934-020-01397-y
            E. Jiménez-Ortega, S. Valenzuela, M. Ramírez-Escudero, F.J. Pastor and J. Sanz-Aparicio."Structural analysis of the reducing-end xylose-releasing exo-oligoxylanase Rex8A from Paenibacillus barcinonensis BP-23 deciphers its molecular specificity." The FEBS Journal, 287, 24, 5362-5374  (2020), doi: 10.1111/febs.15332

            K.V. Mahasenan, M.T. Batuecas, S. Benedetti, Ch. Kim, N. Rana, M. Lee, D. Hesek, J.F. Fisher, J. Sanz-Aparicio, J.A. Hermoso and S. Mobashery."Catalytic Cycle of Glycoside Hydrolase BglX from Pseudomonas aeruginosa and Its Implications for Biofilm Formation". ACS Chemical Biology (2020) 15, 189-196, doi:10.1021/acschembio.9b00754

            P.E. Kidibule, P. Santos-Moriano, F.J. Plou and M. Fernández-Lobato. "Endo-chitinase Chit33 specificity on different chitinolytic materials allows the production of unexplored chitooligosaccharides with antioxidant activity". Biotechnology Reports , 27,  e00500 (2020), doi: 10.1016/j.btre.2020.e00500

            S. Neifar, F.V. Cervantes, H. BenHlima, A. Bouanane-Darenfed, A.O. Ballesteros, F.J. Plou and  S. Bejar. “Immobilization of the glucose isomerase from Caldicoprobacter algeriensis on Sepabeads EC-HA and its efficient application in continuous High Fructose Syrup production using packed bed reactor”Food Chemistry  309,  125710 (2020)doi: 10.1016/j.foodchem.2019.125710
             V. Füreder, B. Rodríguez-Colinas, F.V. Cervantes, L. Fernández-Arrojo, A. Poveda, J. Jiménez-Barbero, A.O. Ballesteros and F.J. Plou."Selective synthesis of galactooligosaccharides containing β(1→3) linkages with  β-galactosidase from Bifidobacterium bifidum (Saphera)"Journal of Agricultural and Food Chemistry 68(17), 4930-4938 (2020) doi: 10.1021/acs.jafc.0c00997.
            S. Alonso, G. Santiago, I. Cea-Rama, L. Fernández-Lopez, C. Coscolin, J. Modregger, A.K. Ressmann, M. Martínez-Martínez, H. Marrero, R. Bargiela, M. Pita, J.L. González-Alfonso, M. Briand, D. Rojo, C. Barbas, F.J. Plou, P.N. Golyshin, P. Shahgaldian, J. Sanz-Aparicio, V. Guallar and M. Ferrer.“Genetically engineered proteins with two active sites for enhanced biocatalysis and synergistic chemo- and biocatalysis" . Nature Catalysis 3(3). 319-328 (2020), doi: 10.1038/s41929-019-0394-4.

            F.V. Cervantes, S. Neifar, Z. Merdzo, J. Viña-González, L. Fernández-Arrojo, A.O. Ballesteros, M. Fernández-Lobato, S. Béjar and F.J. Plou.“A three-step process for the bioconversion of whey permeate into D-tagatose syrup free of glucose”Catalysts 10(6), 647 (2020) doi: 10.3390/catal10060647

        S. Míguez-Amil, E. Jiménez-Ortega, M. Ramírez-Escudero, D. Talens-Perales, J. Marín-Navarro, J. Polaina, J. Sanz-Aparicio, R. Fernández-Leiro. "The cryo-EM structure of Thermotoga maritima β-galactosidase: quaternary structure guides protein engineering"ACS Chemical Biology 15, 1, 179  (2020) doi:10.1021/acschembio.9b00752

        C.  Giunta, I. Cea-Rama, S. Alonso, M.L. Briand, R. Bargiela, C. Coscolín, P.F.X. Corvini, M. Ferrer, J. Sanz-Aparicio and P. Shahgaldian."Tuning the Properties of Natural Promiscuous Enzymes by Engineering Their Nano-environment". ACS NANO, (2020), 14, 17652−17664.  doi:


              M. Ramirez-Escudero, N. Miguez, M. Gimeno-Perez, A.O. Ballesteros, M. Fernandez-Lobato, F.J. Plou and J. Sanz-Aparicio. “Deciphering the molecular specificity of phenolic compounds as inhibitors or glycosyl acceptors of β fructofuranosidase from Xanthophyllomyces dendrorhous. Scientific Reports 9, 17441 (2019), doi:10.1038/s41598-019-53948-y.

              M. García-González, F.V. Cervantes, F.J. Plou, M. Remacha, A. Poveda, J. Jiménez-Barbero and M. Fernández-Lobato. “Efficient production of isomelezitose by a novel glucosyltransferase activity in Metschnikowia reukaufii cell extracts”. Microbial Biotechnology 12, 6, 1274-1285(2019), doi:10.1111/1751-7915.13490

              H. García-Arellano, J. L. González-Alfonso, C. Ubilla, F. Comelles, M. Alcalde, M. Bernabé, J-L Parra, A. O. Ballesteros and F. J. Plou. “Production and surfactant properties of tert-butyl α-D-glucopyranosides catalyzed by cyclodextrin glucanotransferase”. Catalysts  9(7), 575 (2019), doi: 10.3390/catal9070575 .
               P. Molina-Espeja, P. Santos-Moriano, E. García-Ruiz, A.O. Ballesteros, F.J. Plou and M. Alcalde. “Structure-guided immobilization of an evolved unspecific peroxygenase”. International Journal of Molecular Sciences 20(7), 1627 (2019), doi:10.3390/ijms20071627. (This article belongs to the Special Issue «Industrial Enzymes: Structure, Function and Applications»).

              M. Martínez-Bailén, E. Jiménez-Ortega, A.T. Carmona, I. Robina, J. Sanz-Aparicio, D. Talens-Perales, J. Polaina, C. Matassini, F. Cardonad and A.J. Moreno-Vargas. "Structural basis of the inhibition of GH1 β-glucosidases by multivalent pyrrolidine iminosugars"Bioorganic Chemistry 89, 103026-103036 (2019)doi:10.1016/j.bioorg.2019.103026.

              P. Santos-Moriano, P. Kidibule, N. Míguez, L. Fernández-Arrojo, A.O. Ballesteros, M. Fernández-Lobato and F.J. Plou. “Tailored enzymatic synthesis of chitooligosaccharides with different deacetylation degrees and their anti-inflammatory activity”. Catalysts (2019),  9(7), 575 .

              J.L. González-Alfonso, P. Peñalver, A.O. Ballesteros, J.C. Morales and F.J. Plou. “Effect of α-glucosylation on the stability, antioxidant properties, toxicity and neuroprotective activity  of (‒)-epigallocatechin gallate”. Frontiers in Nutrition (2019), doi: 10.3389/fnut.2019.00030

              P. Molina-Espeja, P. Santos-Moriano, E. García-Ruiz, A. Ballesteros, FJ. Plou and M. Alcalde. “Structure-Guided immobilization of an evolved unspecific peroxygenase”. International Journal of Molecular Sciences, 20(7), 1627  (2019)doi:10.3390/ijms20071627 .

              P. Gómez de Santos, F.V. Cervantes, F. Tieves, F. Hollmann, F.J Plou and M. Alcalde.  “Benchmarking of laboratory evolved unspecific peroxygenases for the synthesis of human drug metabolites”.  Tetrahedron 75, 1827-1831 (2019), doi:10.1016/j.tet.2019.02.013.

              M.S. Gutiérrez, S. Campusano, A.M. González, M. Gómez, S. Barahona, D. Sepúlveda, P.J. Espenshade, M. Fernández-Lobato, M. Baeza, V. Cifuentes and J. Alcaíno,  “Sterol Regulatory Element-Binding Protein (Sre1) promotes the synthesis of carotenoids and sterols in Xanthophyllomyces dendrorhous. Frontiers in Microbiology (2019) doi: 10.3389/fmicb.2019.00586.


              J.L. González-Alfonso, N. Míguez, J. D. Padilla, L. Leemans, A. Poveda, J. Jiménez-Barbero, A. O. Ballesteros, G. Sandoval and F. J. Plou.''Optimization of Regioselective Glucosylation of Hesperetin Catalyzed by Cyclodextrin Glucanotransferase'' . Molecules, 23, 2285, (2018) doi: 10.3390/molecules23112885 

              M. Baeza ,M. Fernández-Lobato ,J. Alcaíno andV. Cifuentes . "Isolation and Characterization of Extrachromosomal Double-Stranded RNA Elements from Carotenogenic Yeasts" . Methods Molecular Biology 1852:327-339 (2018)

              J.L. González-Alfonso, L. Casas-Godoy, J. Arrizón, D. Arrieta-Báez, A.O. Ballesteros, G. Sandoval and F.J. Plou. ''Lipase-catalyzed synthesis of fatty acid esters of trisaccharides'' . Methods Molecular Biology, 1835:287-296. (2018), doi:10.1007/978-1-4939-8672-9_15

              D. Rodrigo-Frutos, D. Piedrabuena, J. Sanz-Aparicio and M. Fernández-Lobato. "Yeast cultures expressing the Ffase from Schwanniomyces occidentalis, a simple system to produce the potential prebiotic sugar 6-kestose" Applied Microbiology and Biotechnology, 103, 279-289 (2019), doi:10.1007/s00253-018-9446-y

              P. Santos-Moriano, P.E. Kidibule, E. Alleyne, A.O. Ballesteros, A. Heras, M. Fernández-Lobato and F.J. Plou. "Efficient conversion of chitosan into chitooligosaccharides by a chitosanolytic activity from Bacillus thuringiensis”.  Process Biochemistry 73, 102-108 (2018). doi:10.1016/j.procbio.2018.07.017

              N. Míguez, M. Ramírez-Escudero, M. Gimeno-Pérez, A. Poveda, J. Jiménez-Barbero, A. O. Ballesteros, M. Fernández-Lobato, J. Sanz-Aparicio and F. J. Plou. “Fructosylation of hydroxytyrosol by the β-fructofuranosidase from Xanthophyllomyces dendrorhous: Insights into the molecular basis of the enzyme specificity. ChemCatChem, 10, 21, 4878-4887, (2018). doi:10.1002/cctc.201801171

               J.L. González-Alfonso, L. Leemans, A. Poveda, J. Jiménez-Barbero, A.O. Ballesteros and F.J. Plou. "Efficient α-glucosylation of epigallocatechin gallate catalyzed by cyclodextrin glucanotransferase from Thermoanaerobacter sp." Journal of Agricultural and Food Chemistry 66, 7402–7408 (2018). doi:10.1021/acs.jafc.8b02143

              J.L. González-Alfonso, D. Rodrigo-Frutos, E. Belmonte-Reche, P. Peñalver, A. Poveda, J. Jimenez-Barbero, A.O. Ballesteros, Y. Hirose, J. Polaina, J.C. Morales, M. Fernández-Lobato and F. J. Plou. "Enzymatic synthesis of a novel pterostilbene α-glucoside by the combination of cyclodextrin glucanotransferase and amyloglucosidase". Molecules 23(6): 1271 (2018). doi:10.3390/molecules23061271

              B. Gómez-Fernández, E. García-Ruiz, J. Martin, P. Gómez de Santos, P. Santos-Moriano, F.J. Plou, A. Ballesteros, M. García, M. Rodríguez, V.A. Risso, J.M. Sánchez-Ruiz, S.M. Whitney and M. Alcalde. "Directed -in vitro- evolution of Precambrian and extant Rubiscos". Scientific Reports 8, 532 (2018). doi:10.1038/s41598-018-23869-3

              N. Míguez, M. Gimeno-Pérez, D. Fernández-Polo, F.V. Cervantes, A.O. Ballesteros, M. Fernández-Lobato, M.H. Ribeiro and F.J. Plou. "Immobilization of the β-fructofuranosidase from Xanthophyllomyces dendrorhous by entrapment in polyvinyl alcohol and its application to neo-fructooligosaccharides production". Catalysts 8(5), 201 (2018). doi:10.3390/catal8050201

              A.R. Alcántara, E. García-Junceda, V. Gotor-Santamaría and F.J. Plou. "Biocatalysis in Spain: a field of success and innovation". Biocatalysis and Biotransformation (2018).  Special Issue "Biocatalysis in Spain", doi:10.1080/ 10242422.2017.1420064

              P.E. Kidibule,  P. Santos-Moriano, E. Jiménez-Ortega, M. Ramírez-Escudero, M.C. Limón, M. Remacha, F.J. Plou, J. Sanz-Aparicio and  M. Fernández-Lobato. "Use of chitin and chitosan to produce new chitooligosaccharides by chitinase Chit42: enzymatic activity and structural basis of protein specificity". Microbial Cell Factories 17:47 (2018) doi: 10.1186/s12934-018-0895-x

              P. Santos-Moriano, L. Fernández-Arrojo, M. Mengíbar, E. Belmonte-Reche, P. Peñalver, F.N. Acosta, P. Kidibule, A.O. Ballesteros, J.C. Morales, M. Fernández-Lobato and F.J. Plou. "Enzymatic production of fully deacetylated chitooligosaccharides and their neuroprotective and anti-inflammatory properties". Biocatalysis and Biotransformation 36, 57-67 (2018). Special Issue "Biocatalysis in Spain". doi: 10.1080/10242422.2017.1295231

              M. Ramírez-Escudero, P. Molina-Espeja, P. Gómez de Santos, M. Hofritcher, J. Sanz-Aparicioand M. Alcalde. "Structural insights in the substrate promiscuity of a laboratory-evolved peroxygenase"ACS Chem. Biol. (2018), 13, 3259–3268.  doi:10.1021/acschembio.8b00500


      E. Franco-Echevarría, J. Sanz-Aparicio, C.A. Brearley, J.M. González-Rubio and B. González. "The crystal structure of mammalian inositol 1,3,4,5,6-pentakisphosphate 2-kinase reveals a new zinc-binding site and key features for protein function."Journal of Biology Chemical (2017) 292, 10534-10548 doi: 10.1074/jbc.M117.780395 DIGITAL CSIC

      M. Casa-Villegas, J. Marín-Navarro and J. Polaina. "Synergies in coupled hydrolysis and fermentation of cellulose using a Trichoderma reesei enzyme preparation and a recombinant Saccharomyces cerevisiae strain". World Journal of Microbiology and Biotechnology 33:140 (2017).  doi: 10.1007/s11274-017-2308-4

      A. Rico-Díaz, M. Ramírez-Escudero, A. Vizoso-Vázquez, M.E. Cerdán, M. Becerra and J. Sanz-Aparicio. "Structural features of Aspergillus niger beta-galactosidase define its activity against glycoside linkages"FEBS Journal 284,1815-1829 (2017). doi:10.1111/febs.14083

      L. Deladino, A. Schneider-Teixeira, F.J. Plou, A.S. Navarro and A.D. Molina-García. "Effect of high hydrostatic pressure, alkaline and combined treatments on corn starch granules metal binding: structure, swelling behavior and thermal properties assessment". Food and Bioproducts Processing 102, 241-249 (2017).  doi:10.1016/j.fbp.2017.01.003 

      M. Nieto-Domínguez, L.I. de Eugenio, M.J. York-Durán, B. Rodríguez-Colinas, F.J Plou, E. Chenoll, E. Pardo, F. Codoñer and M.J. Martínez. “Prebiotic effect of xylooligosaccharides produced from birchwood xylan by a novel fungal GH11 xylanase”. Food Chemistry 232, 105-113 (2017), doi:10.1016/j.foodchem.2017.03.149

      M. Casa-Villegas, J. Marín-Navarro and  J. Polaina. "Synthesis of isomaltooligosaccharides by Saccharomyces cerevisiae cells expressing Aspergillus niger alpha-glucosidases". ACS Omega 2, 11, 8062-8068 (2017)doi:10.1021/acsomega.7b01189 

    C.V. Yáñez-Ñeco, B. Rodríguez-Colinas, L. Amaya-Delgado, A.O. Ballesteros, A. Gschaedler, F.J. Plou and J. Arrizón. "Galactooligosaccharide production from Pantoea anthophila strains isolated from “Tejuino”, a Mexican traditional fermented beverage". Catalysts 7(8), 242 (2017). doi:10.3390/catal7080242

    D. Talens-Perales, J. Marín-Navarro, D. Garrido, E. Almansa and J. Polaina. "Fixation of bioactive compounds to the cuticle of Artemia". Aquaculture 474,  95–100 (2017). doi:10.1016/j.aquaculture.2017.03.044

    M. Nieto-Domínguez, L.I. de Eugenio, P. Peñalver, E. Belmonte-Reche, J.C. Morales, A. Poveda, J. Jiménez-Barbero, A. Prieto, F.J. Plou and M.J. Martínez. "Enzymatic synthesis of a novel neuroprotective hydroxytyrosol glycoside". Journal of Agricultural and Food Chemistry 65, 10526-10533 (2017), doi:10.1021/acs.jafc.7b04176


    S.V. Valenzuela, S. López, P. Biely, J. Sanz-Aparicio and J. Pastor. "The glycoside hydrolase family 8 reducing-end xylose-releasing exo-oligoxylanase Rex8a from Paenibacillus barcinonensis BP-23 is active on branched xylooligosaccharides"Appl. Env. Microbiol. 82, 5116-5124 (2016). doi:10.1128/AEM.01329-16.

    B. Rodríguez-Colinas, L. Fernández-Arrojo, P. Santos-Moriano, A.O. Ballesteros and F.J. Plou. “Continuous packed bed reactor with immobilized β-galactosidase for production of galactooligosaccharides (GOS)”. Catalysts 6(12), 189 (2016). Special Issue "Immobilized Enzymes: Strategies for Enzyme Stabilization". doi:10.3390/catal6120189

    D. Talens-Perales, A. Górska, D.H. Huson, J. Polaina and J. Marin-
    Navarro. "Analysis of Domain Architecture and Phylogenetics of Family 2 Glycoside Hydrolases (GH2)". PLOS ONE, December, 1-17 (2016)

    P. Córdova,  J. Alcaíno, N. Bravo, S. Barahona, D. Sepúlveda, M. Fernández-Lobato, M.Baeza and V. Cifuentes. “Regulation of carotenogenesis in the red yeast Xanthophyllomyces dendrorhous: the role of the transcriptional co-repressor complex Cyc8-Tup1 involved in catabolic repression”. Microbial Cell Factories  15(1):193 (2016) doi:10.1186/s12934-016-0597-1Open access

    M. Ramírez-Escudero, M.V. del Pozo, J. Marín-Navarro, B. González, P.N. Golyshin, J. Polaina, M. Ferrer and J. Sanz-Aparicio. "Structural and functional characterization of a ruminal β-glycosidase defines a novel subfamily of glycosyl hydrolase family 3 with permuted domain topology"Journal of Biological Chemistry 291(46):24200-24214 (2016) doi:10.1074/jbc.M116.747527

    I. Saugar, B. Molloy, E. Sanz, M.B. Sánchez, M. Fernández-Lobato and A. Jiménez. "Characterization of the biosynthetic gene cluster (ata) for the A201A aminonucleoside antibiotic from Saccharothrix mutabilis subsp. capreolus". J Antibiot (Tokyo) 70(4):404-413 (2016doi: 10.1038/ja.2016.123

    P. Santos-Moriano, J.M. Woodley and F.J. Plou. "Continuous production of chito-oligosaccharides by an immobilized enzyme in a dual-reactor system"Journal of Molecular Catalysis B: Enzymatic 133, 211-217 (2016)doi:10.1016/j.molcatb.2016.09.001

    P. Santos-Moriano, L. Monsalve-Ledesma, M. Ortega-Muñoz, L. Fernández-Arrojo, A.O. Ballesteros, F. Santoyo-González, F.J. Plou. "Vinyl sulfone-activated silica for efficient covalent immobilization of alkaline unstable enzymes: application to levansucrase for fructooligosaccharides synthesis". RSC Advances 6, 64175-64181 (2016). doi: 10.1039/C6RA14046G 

    J. Alcaíno , N. Bravo , P. Córdova, A.E. Marcoleta, G. Contreras, S. Barahona, D. Sepúlveda, M. Fernández-Lobato, M. Baeza and V. Cifuentes. "The involvement of Mig1 from Xanthophyllomyces dendrorhous in catabolic repression: An active mechanism contributing to the regulation of carotenoid production". PLOS ONE 11(9):e0162838 (2016). doi:10.1371/journal.pone.0162838 Open access

    D. Piedrabuena, N. Míguez, A. Poveda, F.J. Plou, M. Fernández-Lobato. “Exploring the transferase activity of Ffase from Schwanniomyces occidentalis, a β-fructofuranosidase showing high fructosyl-acceptor promiscuity”. Applied Microbiology and Biotechnology 100(20), 8769-8778 (2016). doi:10.1007/s00253-016-7628-z  

    M. Ramírez-Escudero, M. Gimeno-Pérez, B. González, D. Linde, Z. Merzdo, M. Fernández-Lobato and J. Sanz-Aparicio. "Structural analysis of β-fructofuranosidase from Xanthophyllomyces dendrorhous reveals unique features and the crucial role of N-glycosylation in oligomerization and activity". Journal of Biological Chemistry 291, 6843-6857 (2016). doi: jbc.M115.708495

    D. Talens-Perales, J. Polaina and J. Marín-Navarro. "Structural dissection of the active site of Thermotoga maritima β‑galactosidase identifies key residues for transglycosylating activity".  Journal of Agriculture and Food Chemistry  64, 2917−2924 (2016). doi: 10.1021/acs.jafc.6b00222

    L. Casas-Godoy, J. Arrizón, D. Arrieta, F.J. Plou and G. Sandoval. “Synthesis and emulsifying properties of carbohydrate fatty acid esters produced from Agave tequilana fructans by enzymatic acylation”. Food Chemistry 204, 437-443 (2016). doi:10.1016/j.foodchem.2016.02.153 

    P. Gutiérrez-Alonso, M. Gimeno-Pérez, M. Ramírez-Escudero, F.J. Plou, J. Sanz-Aparicio and M. Fernández-Lobato. “Molecular characterization and heterologous expression of a Xanthophyllomyces dendrorhous α-glucosidase with potential for prebiotics production”Applied Microbiology and Biotechnology 100(7), 3125-3135

    P. Zambelli, L. Tamborini, S. Cazzamalli, A. Pinto, S. Arioli, S. Balzaretti, F.J. Plou, L. Fernández-Arrojo, F. Molinari, P. Conti and D. Romano. “An efficient continuous flow process for the synthesis of a non-conventional mixture of fructooligosaccharides”Food Chemistry 190, 607-613 (2016)  doi:10.1016/j.foodchem.2015.06.002


    J. Marín-Navarro, N. Roupain, D. Talens-Perales and J. Polaina. "Identification and structural analysis of amino acid substitutions that increase the stability and activity of Aspergillus niger glucose oxidase". PLOS ONE 10(12): e0144289 (2015) doi: 10.1371/journal.pone.0144289
      Open access

    P. Santos-Moriano, L. Fernández-Arrojo, A. Poveda, J. Jiménez-Barbero, A.O. Ballesteros and F.J. Plou. “Levan versus fructooligosaccharide synthesis using the levansucrase from Zymomonas mobilis: effect of reaction conditions”. Journal of Molecular Catalysis B: Enzymatic, 119, 18–25 (2015), doi:10.1016/j.molcatb.2015.05.011 

    M.A. Sainz-Polo, B. González, M. Menéndez, F.I.J. Pastor and J. Sanz-Aparicio. "Exploring Multimodularity in Plant Cell Wall Deconstruction: Structural and Functional Analysis of Xyn10C Containing the CBM22-1-CBM22-2 Tandem". Journal of Biological Chemistry 290(28):17116-30 (2015), doi:10.1074/jbc.M115.659300

    P. Zambelli, I. Serra, L. Fernández-Arrojo, F.J. Plou, L. Tamborini, P. Conti, M.I. Contente, F. Molinari and D. Romano. “Sweet-and-salty biocatalysis: fructooligosaccharides production using Cladosporium cladosporioides in seawater”. Process Biochemistry 50, 1086-1090 (2015), doi:10.1016/j.procbio.2015.04.006 

    M.A. Sainz-Polo, B. González-Pérez, F.J. Pastor and J. Sanz-Aparicio."Crystallization and preliminary X-ray diffraction analysis of the N-terminal domain of Paenibacillus barcinonensis xylanase 10C containing the CBM22-1--CBM22-2 tandem". Acta Crystallogr. F71, 136-140 (2015). doi:10.1107/S2053230X14027496 

    J. Marín-Navarro, D. Talens and J. Polaina. "One-pot production of fructooligosaccharides by a Saccharomyces cerevisiae strain expressing an engineered invertase". Applied Microbiology and Biotechnology 99(6):2549-2555

    P. Santos-Moriano, L. Fernández-Arrojo, B. Rodríguez-Colinas, A.O. Ballesteros and F.J. Plou. "Enzymatic synthesis of fructooligosaccharides that stimulate the gut microbiota". Anales de la Real Academia Nacional de Farmacia  81, 48-62 (2015). Download

    J. Sanz-Aparicio. “The legacy of women to crystallography”. Arbor 191 (772): a223 (2015). doi:10.3989/arbor.2015.772n2002. Special Issue "Celebrating 100 years of modern crystallography" (Mancheño, J.M., Ed..)

    L. Fernández-Arrojo, P. Santos-Moriano, B. Rodríguez-Colinas, A.O. Ballesteros and F.J. Plou. "Micro-scale procedure for enzyme immobilization screening and operational stability assays". Biotechnology Letters 37, 1593-1600 

    N. Corzo, J. L. Alonso, F. Azpiroz, M. A. Calvo, M. Cirici, R. Leis, F. Lombó, I. Mateos-Aparicio, F. J. Plou, P. Ruas-Madiedo, P. Ruperez, A. Redondo-Cuenca, M. L. Sanz and A. Clemente. "Prebióticos; concepto, propiedades y efectos beneficiosos". Nutrición Hospitalaria 31 (Supl. 1), 99-118 (2015). Download   

    M. Gimeno, D. Linde, L. Fernández-Arrojo, F.J. Plou and M. Fernández Lobato. "Heterologous over-production of β-fructofuranosidase from yeast Xanthophyllomyces dendrorhous, an enzyme producing prebiotic sugars". Applied Microbiology and Biotechnology 99, 3459-3467 (2015),


    P. Zambelli, L. Fernández-Arrojo, D. Romano, P. Santos-Moriano, M. Gimeno-Pérez, A. Poveda, R. Gandolfi, M. Fernández-Lobato, F. Molinari and F.J. Plou. "Production of fructooligosaccharides by mycelium-bound transfructosylation activity present in Cladosporium cladosporioides and Penicilium sizovae". Process Biochemistry 49, 2174-2180 (2014).   

    B. Rodríguez-Colinas, L. Fernández-Arrojo, A.O. Ballesteros and F.J. Plou. "Galactooligosaccharides formation during enzymatic hydrolysis of lactose: towards a prebiotic enriched milk".  Food Chemistry, 145, 388-394 (2014)  Postprint Digital CSIC

    M.A Sainz-Polo, S. Valenzuela, B. González-Pérez, F.J. Pastor and J. Sanz-Aparicio. "Structural analysis of glucuronoxylan specific Xyn30D and its attached CBM35 domain give insights into the role of modularity in specificity". Journal of Biological Chemistry 289, 31088-31101 (2014).

    A. Rico-Díaz, A. Vizoso-Vázquez, M.A. Cerdán, M. Becerra and J. Sanz-Aparicio. "Crystallization and preliminary X-ray diffraction data of β-galactosidase from Aspergillus niger"Acta Crystallogr F70, 1529-1531 (2014)

    J.P. Castruita-Domínguez, S.E. González-Hernández, J. Polaina, L.L. Flores-Villavicencio, A. Alvarez-Vargas, A. Flores-Martínez, P. Ponce-Noyola and C.A.Leal-Morales. "Analysis of a polygalacturonase gene of Ustilago maydis and characterization of the encoded enzyme". Journal of Basic Microbiology 54, 340-349 (2014).

    J. Marín-Navarro, D. Talens-Perales, A. Oude-Vrielink, F.J. Cañada and J. Polaina. "Immobilization of thermostable β-galactosidase on epoxy support and its use for lactose hydrolysis and galactooligosaccharides biosynthesis"World Journal of Microbiology and Biotechnology 30989-998 (2014).

    M.A. Sainz-Polo, S.V. Valenzuela, F.J. Pastor and J. Sanz-Aparicio. "Crystallization and preliminary X-ray diffraction analysis of Xyn30D from Paenibacillus barcinonensis". Acta Crystallographica Section F: Structural Biology Communications 70, 963-966 (2014).
    M. Gimeno-Pérez, P. Santos-Moriano, L. Fernández-Arrojo, A. Poveda, J. Jiménez-Barbero, A.O. Ballesteros, M. Fernández-Lobato and F.J. Plou. "Regioselective synthesis of neo-erlose by the β-fructofuranosidase from Xanthophyllomyces dendrorhous"Process Biochemistry 49, 423-429 (2014). http:/ 


    J.P.Castruita-Domínguez ,S.E. González-Hernández ,J. Polaina ,L.L. Flores-Villavicencio ,A. Álvarez-Vargas ,A. Flores-Martínez ,P. Ponce-Noyola and C.A. Leal-Morales . "Analysis of a polygalacturonase gene of Ustilago maydis and characterization of the encoded enzyme". Journal of Basic Microbiology (2013). 

    M. Álvaro-Benito, M. Fernández-Lobato, K. Baronian and G. Kunze. "Assessment of Schwanniomyces occidentalis as a host for protein production using the wide-range Xplor expression platform". Applied Microbiology and Biotechnology , 97, 4443 - 4456 (2013)doi: 10.1007/s00253-012-4527-9

    M. de Abreu, M. Álvaro-Benito, J. Sanz-Aparicio, F.J. Plou, M. Fernández-Lobato and M. Alcalde. "Synthesis of 6-kestose improved by a highly efficient β-fructofuranosidase engineered by directed evolution". Advanced Synthesis & Catalysis, 355, 1698-1702 (2013) doi:10.1002/adsc.201200769. 

    L. Fernández-Arrojo, B. Rodríguez-Colinas, P. Gutiérrez-Alonso, M. Fernández-Lobato, M. Alcalde, A.O. Ballesteros and F.J. Plou. "Dried alginate-entrapped enzymes (DALGEEs) and their application to the production of fructooligosaccharides". Process Biochemistry, in press accepted manuscript(2013). doi:10.1016/j.procbio.2013.02.015 

    P. Urrutia, B. Rodríguez-Colinas, L. Fernández-Arrojo, A.O. Ballesteros, L. Wilson, A. Illanes and F.J. Plou. "Detailed analysis of galactooligosaccharides synthesis with β-galactosidase from Aspergillus oryzae". Journal of Agricultural and Food Chemistry61, 1081–1087 (2013). doi: 10.1021/jf304354u

    M.A. Sainz-Polo, M. Ramírez, A. Lafraya, B. González, J. Marín-Navarro, J. Polaina and J. Sanz-Aparicio. "The three-dimensional structure of Saccharomyces invertase: role of a non-catalytic domain in oligomerization and substrate specificity"Journal of Biological Chemistry288, 9755-9766 (2013)  doi:10.1074/jbc.M112.446435. 

    B. Rodríguez-Colinas, S. Kolida, M. Baran, A.O. Ballesteros, R.A. Rastall and F.J. Plou. "Analysis of fermentation selectivity of purified galacto-oligosaccharides by in vitro human faecal fermentation". Applied Microbiology and Biotechnology, 97, 5743-5752 (2013) doi: 10.1007/s00253-013-4892-z


    M. Álvaro-Benito, M.A. Sainz-Polo, D. Gonáalez-Pérez, B. González, F.J. Plou, M. Fernández-Lobato and J. Sanz-Aparicio. "Structural and kinetic insights reveal that the amino acid pair Gln-228/Asn-254 modulates the transfructosylating specificity of Schwanniomyces occidentalis β-fructofuranosidase, an enzyme that produces prebiotics". Journal of Biological Chemistry, 287, 19674-19686, (2012), doi:10.1074/jbc.M112.355503.

    M.A. Sainz-Polo, A. Lafraya, A. Polo, J. Marín-Navarro, J. Polaina and J. Sanz-Aparicio. "Crystallization and preliminary X-ray diffraction analysis of the invertase from Saccharomyces cerevisiae"Acta Crystallographica Section F: Structural Biology and Crystallization Communications, Vol. 68, 1538-1541 (2012) doi: 10.1107/S1744309112044417 

    D. Linde, B. Rodríguez-Colinas, M. Estévez, A. Poveda, F.J. Plou and M. Fernández-Lobato. "Analysis of neofructooligosaccharides production mediated by the extracellular beta-fructofuranosidase from Xanthophyllomyces dendrorhous". Bioresource Technology, 23,123-130(2012) doi: 10.1016/j.biortech.2012.01.023 

    A. Pereira-Rodríguez, R. Fernández-Leiro, M.I. González-Siso, M. Esperanza Cerdán, M. Becerra and J. Sanz-Aparicio. "Structural basis of specificity in tetrameric Kluyveromyces lactis β-galactosidase". Journal of Structural Biology (2012) 177, 392–401.  doi:10.1016/j.jsb.2011.11.031 

    B. Rodríguez-Colinas, A. Poveda, J. Jiménez-Barbero, A.O. Ballesteros and F.J. Plou. "Galacto-oligosaccharide synthesis from lactose solution or skim milk using the β-galactosidase from Bacillus circulans". Journal of Agricultural and Food Chemistry60, 6391-6398 (2012)

    M. Camarillo-Cadena ,G. Garza-Ramos ,M. Peimbert ,J. Polaina ,G. Pérez-Hernández and R.A. Zubillaga . "Additive effect of single amino acid replacements on the kinetic stability of ß-glucosidase B"The Protein Journal 31:615-622. (2012) doi: 10.1007/s10930-012-9445-2

    M.V. Del Pozo , L. Fernández-Arrojo , J. Gil-Martínez , A Montesinos , T.N. Chernikova , T.Y. Nechitaylo , A. Waliszek , M. Tortajada , A Rojas , S.A. Huws , O.V. Golyshina , C.J. Newbold , J. Polaina , M. Ferrer and P.N. Golyshin . "Microbial β-glucosidases from cow rumen metagenome enhance the saccharification of lignocellulose in combination with commercial cellulase cocktail".Biotechnol Biofuels. 5(1):73 (2012). doi: 10.1186/1754-6834-5-73. 

    N.R. Aimaretti, C.V. Ybalo, M.L. Rojas, F.J. Plou and J.C. Yori. "Production of bioethanol from carrot discards". Bioresource Technology123, 727-732(2012). doi:10.1016/j.biortech.2012.08.035. 

    M. Ferrer , A. Ghazi , A. Beloqui , J.M. Vieites , N. López-Cortés , J. Marín-Navarro , T.Y. Nechitaylo , M.E. Guazzaroni , J. Polaina , A. Waliczek , T.N. Chernikova, O.N. Reva, O.V. Golyshina and P.N. Golyshin . "Functional metagenomics unveils a multifunctional glycosyl hydrolase from the family 43 catalysing the breakdown of plant polymers in the calf rumen"PLoS One 7(6):e38134 (2012)  doi:10.1371/journal.pone.0038134 


    B. Rodríguez-Colinas, M. de Abreu, L. Fernández-Arrojo, R. de Beer, A. Poveda, J. Jimeéez-Barbero, D. Haltrich, A. Ballesteros, M. Fernández-Lobato and F.J. Plou. "Production of galacto-oligosaccharides by the beta-galactosidase from Kluyveromyces lactis: comparative analysis of permeabilized cells versus soluble enzyme". Journal of Agricultural and Food Chemistry, 59, 10477-10484 (2011) doi:10.1021/jf2022012

    A. Lafraya, J. Sanz-Aparicio, J. Polaina and J. Marín-Navarro . "Fructo-oligosaccharide synthesis by mutant versions of Saccharomyces cerevisiae invertase". Applied and Environmental Microbiology 77 (17) , 6148-6157 (2011)doi: 10.1128/AEM.05032-11

    M.A. de Abreu, M. Álvaro-Benito, F.J. Plou, M. Fernández-Lobato and M. Alcalde. "Screening beta-fructofuranosidases mutant libraries to enhance the transglycosylation rates of beta-(2→6) fructooligosaccharides".Combinatorial Chemistry & High-Throughput Screening, 14, 730-738 (2011)doi:10.2174/138620711796504370 

    J. Marín-Navarro and J. Polaina . "Glucoamylases: structural and biotechnological aspects" Applied Microbiology and Biotechnology. 89: 1267-1273 (2011) doi:10.1007/s00253-010-3034-0 

    P. Torres-Salas, A. del Monte-Martinez, B. Cutiño-Ávila, B. Rodríguez-Colinas, M. Alcalde, A. O. Ballesteros and F. J. Plou. "Immobilized biocatalysts: novel approaches and tools for binding enzymes to supports". Advanced Materials, 23, 5275–5282 (2011) doi:10.1002/adma.201101821

    L. Gurgu, A. Lafraya, J. Polaina and J. Marín-Navarro. "Fermentation of cellobiose to ethanol by industrial Saccharomyces strains carrying the β-glucosidase gene (BGL1) from Saccharomycopsis fibuligera".Bioresource Technology, 102, 5229-5236 (2011)doi:10.1016/j.biortech.2011.01.062 

    C. Guerrero, C. Vera, F.J. Plou and A. Illanes. "Influence of reaction conditions on the selectivity of the synthesis of lactulose with microbial β-galactosidases". Journal of Molecular Catalysis B: Enzymatic, 72, 206-212 (2011) doi:10.1016/j.molcatb.2011.06.007 

    J. Marín-Navarro , L. Gurgu, S. Alamar and J. Polaina. "Structural and functional analysis of hybrid enzymes generated by domain shuffling between Saccharomyces cerevisiae (var. diastaticus) Sta1 glucoamylase andSaccharomycopsis fibuligera Bgl1 ß-glucosidase". Applied Microbiology and Biotechnology 89:121-130 (2011) doi:10.1007/s00253-010-2845-3 


    O. Gallardo , F.I.J. Pastor , J. Polaina , P. Díaz, P. Isorna , B. Gonzalez-Perez and J. Sanz-Aparicio. "Structural insights into the specificity of Xyn10B fromPaenibacillus barcinonensis and its improved stability by forced protein evolution". Journal of Biological Chemistry 285, 2721-2733 (2010).doi:10.1074/jbc.M109.064394 

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