30 March, 2015

The overproduction of the β-fructofuranosidase from Xanthophyllomyces dendrorhous in Pichia pastoris was
successfully achieved. This enzyme is the largest microbial enzyme producing neo-fructooligosaccharides (neo-FOS)
known to date. This achievement is essential to carry
out future mutational analysis of this enzyme in order to
clarify the determinants of its specificity and to perform its
operational improvement using molecular bioengineering
Ref: Applied Microbiology and Biotechnology Vol. 99, pp. 3459-3467 (2015)DOI 10.1007/s00253-014-6145-1