The α-glucosidase from
Schwanniomyces occidentalis (GAM1p) was expressed in
Komagataella phaffii to about 70 mg/L, and its transferase activity studied in detail. Several isomaltooligosaccharides (IMOS) were formed using 200 g/L maltose. The major production of IMOS (81.3 g/L) was obtained when 98% maltose was hydrolysed, of which 34.8 g/L corresponded to isomaltose, 26.9 g/L to isomaltotriose, and 19.6 g/L to panose. In addition, the potential of this enzyme to glycosylate 12 possible hydroxylated acceptors, including eight sugars and four phenolic compounds, was evaluated. Among them, only sucrose, xylose, and piceid (a monoglucosylated derivative of resveratrol) were glucosylated, and the main synthesised products were purified and characterised by MS and NMR.
Ref: “Insights into the transglucosylation activity of α-glucosidase from Schwanniomyces occidentalis”. Zoran Merdzo, Egle Narmontaite, Jose L. Gonzalez-Alfonso, Ana Poveda, Jesus Jimenez-Barbero, Francisco J. Plou, María Fernández-Lobato. Applied Microbiology and Biotechnology 108, 443 (2024). https://doi.org/10.1007/s00253-024-13262-8
